| First Author | Murata T | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 6610 |
| PubMed ID | 29700333 | Mgi Jnum | J:346821 |
| Mgi Id | MGI:6163280 | Doi | 10.1038/s41598-018-24899-7 |
| Citation | Murata T, et al. (2018) Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification. Sci Rep 8(1):6610 |
| abstractText | Epidermal keratinocytes achieve sequential differentiation from basal to granular layers, and undergo a specific programmed cell death, cornification, to form an indispensable barrier of the body. Although elevation of the cytoplasmic calcium ion concentration ([Ca(2+)]i) is one of the factors predicted to regulate cornification, the dynamics of [Ca(2+)]i in epidermal keratinocytes is largely unknown. Here using intravital imaging, we captured the dynamics of [Ca(2+)]i in mouse skin. [Ca(2+)]i was elevated in basal cells on the second time scale in three spatiotemporally distinct patterns. The transient elevation of [Ca(2+)]i also occurred at the most apical granular layer at a single cell level, and lasted for approximately 40 min. The transient elevation of [Ca(2+)]i at the granular layer was followed by cornification, which was completed within 10 min. This study demonstrates the tightly regulated elevation of [Ca(2+)]i preceding the cornification of epidermal keratinocytes, providing possible clues to the mechanisms of cornification. |
