Primary Identifier | MGI:6188018 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Malat1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The complete 6,982 bp of coding sequence, including 250 nucleotides upstream of the transcriptional start site and 321 nucleotides following the 3'-end of the transcript, was flanked by loxP sites. A FRT-flanked neomycin cassette was inserted downstream of the coding sequence but upstream of the second loxP site. Cre-mediated recombination removed the entire floxed region. qRT-PCR confirmed absence of transcripts in various organs. |