| First Author | Jin JP | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 214 |
| Issue | 3 | Pages | 1168-74 |
| PubMed ID | 7575526 | Mgi Jnum | J:28929 |
| Mgi Id | MGI:76466 | Doi | 10.1006/bbrc.1995.2409 |
| Citation | Jin JP, et al. (1995) An embryonic alternative transcription initiation site and the 5'-upstream structure of mouse cardiac troponin T gene. Biochem Biophys Res Commun 214(3):1168-74 |
| abstractText | By specific antibody screening of a lambda ZAPII expression library, we identified a class of cDNAs derived from mouse cardiac troponin T (TnT) mRNA with long 5'-ends containing the -30 TATA sequence of the promoter. These long mRNAs indicated the presence of a novel upstream transcription initiation site in the cardiac TnT gene. Primer extension mapping of the mouse cardiac TnT mRNA confirmed an alternative transcription initiation site 55-base pairs upstream of the major initiation site. The upstream initiation site was found significantly more active in the embryonic/neonatal hearts. Genomic cloning and sequencing further revealed an 1.5-kb 5'-upstream sequence of the mouse cardiac TnT gene and relationships between the promoter regulatory elements and the alternative transcription initiation sites. |
