| Primary Identifier | MGI:6108181 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Hyal3 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A neomycin resistance cassette (neo) was inserted into exon 2 of the gene at the NsiI site. This targeting construct was designed to disrupt Hyal3 while having minimal impact on an overlapping gene, Nat6 (N-acetyltransferase 6), which second exon is embedded within the first intron of this gene. RT-PCR analysis showed that no wild type transcript is expressed from this allele, and that Fus2 trasncripts are not altered in testis, but the levels are reduced about 20% in liver. |
