| First Author | Deng F | Year | 2018 |
| Journal | Cell Death Dis | Volume | 9 |
| Issue | 2 | Pages | 153 |
| PubMed ID | 29396428 | Mgi Jnum | J:314994 |
| Mgi Id | MGI:6829397 | Doi | 10.1038/s41419-017-0244-8 |
| Citation | Deng F, et al. (2018) YAP triggers the Wnt/beta-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury. Cell Death Dis 9(2):153 |
| abstractText | Impaired epithelial regeneration is a crucial pathophysiological feature of ulcerative colitis (UC). Yes-associated protein (YAP1) appears to control cell proliferation and differentiation. In this study, we sought to identify the roles of YAP in intestinal epithelial cell (IEC) self-renewal, regeneration and tumorigenesis. We first observed that YAP was significantly reduced in 62.5% (45/72) of human UC tissues and it was dramatically enhanced during epithelial regeneration in a murine colitis model. Using lentiviral infection, we established a YAP-overexpression (YAP(WT)) mouse model. We then found that after tissue injury, YAP(WT) mice had increased epithelial cell self-renewal capacity and drastically restored intestinal crypt structure. Strikingly, these mice were more susceptible to colitis-associated cancer (CAC) in chemically induced carcinoma. Mechanistically, YAP and beta-catenin showed increased nuclear co-localization during regeneration after inflammation. Overexpressing YAP significantly improved IEC 'wound-healing' ability and increased the expression of both beta-catenin and the transcriptional targets of Wnt signalling Lgr5 and cyclin D1, whereas silencing beta-catenin in YAP(WT) cells attenuated this effect. Remarkably, we observed that YAP could directly interact with beta-catenin in the nucleus and formed a transcriptional YAP/beta-catenin/TCF4 complex; Lgr5 and cyclin D1 were confirmed to be the target genes of this complex. In contrast, cancer cell proliferation and tumour development were suppressed by the phospho-mimetic YAP mutant. In summary, nuclear YAP-driven IEC proliferation could control epithelial regeneration after inflammation and may serve as a potential therapeutic target in UC. However, excessive YAP activation promoted CAC development. |
