| Primary Identifier | MGI:3524978 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Tg(tetORo1-lacZ)3Conk |
| Strain of Origin | FVB/N | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| description | On an FVB/N genetic background, transgenic mice also carrying Tg(Myh6-tTA)6Smbf express Ro1 in the heart. Administration of the drug spiradoline results in a dramatic decrease of heart rate in these mice. X-gal staining of tissues from these mice reveals the pattern of cells that the tet system is working in. Ro1 activates Gi-signaling in response to spiradoline. |
| molecularNote | Two transgenes controlled by the tetO (responsive to tet-transactivator) promoter, one containing "Ro1" and the other containing lacZ, were co-integrated so that beta-galactosidase could be used as a marker for expression. Ro1 is a prototype RASSL (Receptor Activated Solely by a Synthetic Ligand) with an N-terminal FLAG epitope that is extra-cellularly expressed and can be detected with the FLAG monoclonal antibody. Ro1 (RASSL, opioid, #1) is derived from a Kappa Opioid Receptor (G protein-coupled receptor) that was altered to reduce the activation by endogenous hormones, but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. |
