Primary Identifier | MGI:5662417 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Camk2a |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted 60 bp upstream of exon 2 and a floxed neo cassette was inserted approximately 1.2 kbp downstream of exon 2 via homologous recombination. Cre mediated recombination removed the neo cassette leaving exon 2 floxed. Western blot analysis revealed no detectable alteration of protein levels in the brain of homozygous mutant mice. |