| First Author | Martin-DeLeon PA | Year | 1985 |
| Journal | Cytogenet Cell Genet | Volume | 39 |
| Issue | 2 | Pages | 87-92 |
| PubMed ID | 4006523 | Mgi Jnum | J:7893 |
| Mgi Id | MGI:56362 | Doi | 10.1159/000132113 |
| Citation | Martin-DeLeon PA, et al. (1985) Localization of glucose-6-phosphate dehydrogenase in mouse and man by in situ hybridization: evidence for a single locus and transposition of homologous X-linked genes. Cytogenet Cell Genet 39(2):87-92 |
| abstractText | By hybridizing a tritiated human genomic probe (pGD3) to metaphase chromosomes in situ, we have localized the gene for glucose-6-phosphate dehydrogenase (G6PD) in both the human and mouse complement. The locus on the intact human X chromosome is close to the telomere on the long arm, confirming the assignment based on studies of an X/autosome translocation in human-mouse hybrids. Although the signal:background ratio was reduced for the heterologous hybridization of the human probe to mouse metaphases, 20% of the grains were on the X chromosome and 93% of these were in the A region, relatively close to the centromere. The location of G6PD in mouse and man reflects intrachromosomal transposition of these homologous X loci. Genomic DNAs from mouse and man and from hybrids with human X/autosome translocations were digested with several restriction enzymes including EcoRI, PstI, and HpaII, and Southern blots were probed with 32P-pGD3. The results of the analysis also confirm the human G6PD assignment and are consistent with a single copy of the locus in the haploid genome of both species. |
