| First Author | Moore AM | Year | 2018 |
| Journal | Endocrinology | Volume | 159 |
| Issue | 9 | Pages | 3200-3208 |
| PubMed ID | 30010812 | Mgi Jnum | J:264276 |
| Mgi Id | MGI:6195434 | Doi | 10.1210/en.2018-00505 |
| Citation | Moore AM, et al. (2018) Synaptic Innervation of the GnRH Neuron Distal Dendron in Female Mice. Endocrinology 159(9):3200-3208 |
| abstractText | GnRH neuron cell bodies are scattered throughout the basal forebrain but funnel their projections to the median eminence to release GnRH into the pituitary portal system to control fertility. Prior studies have shown that GnRH neurons located in the anterior hypothalamus send projections to the median eminence that have characteristics of both dendrites and axons. These unusual structures have been termed "dendrons." To address whether the dendron is unique to anterior hypothalamic GnRH neurons or is also a characteristic of more rostral GnRH neurons, we used viral vectormediated GnRH neuronspecific tract-tracing coupled with CLARITY optical clearing. Individual rostral preoptic area GnRH neurons in female mice were identified to elaborate processes up to 4 mm in length that exhibited spines and projected all the way to the median eminence before branching into multiple short axons. The synaptic innervation patterns of distal GnRH neuron dendrons and their short axons in the vicinity of the median eminence were examined using electron microscopy. This revealed the presence of a high density of synaptic inputs to distal dendrons at the border of the median eminence. In contrast, no synapses were detected on any GnRH neuron axons. These studies demonstrate that GnRH neurons in the rostral preoptic area project dendrons to the edge of the median eminence, whereupon they branch into multiple short axons responsible for GnRH secretion. The dense synaptic innervation of these distal dendrons likely represents an efficient mechanism for controlling GnRH secretion required for fertility. |
