| First Author | Moradi M | Year | 2017 |
| Journal | J Cell Biol | Volume | 216 |
| Issue | 3 | Pages | 793-814 |
| PubMed ID | 28246119 | Mgi Jnum | J:249820 |
| Mgi Id | MGI:5920402 | Doi | 10.1083/jcb.201604117 |
| Citation | Moradi M, et al. (2017) Differential roles of alpha-, beta-, and gamma-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol 216(3):793-814 |
| abstractText | Axonal branching and terminal arborization are fundamental events during the establishment of synaptic connectivity. They are triggered by assembly of actin filaments along axon shafts giving rise to filopodia. The specific contribution of the three actin isoforms, Actalpha, Actbeta, and Actgamma, to filopodia stability and dynamics during this process is not well understood. Here, we report that Actalpha, Actbeta, and Actgamma isoforms are expressed in primary mouse motoneurons and their transcripts are translocated into axons. shRNA-mediated depletion of Actalpha reduces axonal filopodia dynamics and disturbs collateral branch formation. Knockdown of Actbeta reduces dynamic movements of growth cone filopodia and impairs presynaptic differentiation. Ablation of Actbeta or Actgamma leads to compensatory up-regulation of the two other isoforms, which allows maintenance of total actin levels and preserves F-actin polymerization. Collectively, our data provide evidence for specific roles of different actin isoforms in spatial regulation of actin dynamics and stability in axons of developing motoneurons. |
