Primary Identifier | MGI:6720927 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready, Recombinase | Gene | Vim |
Strain of Origin | C57BL/6J | Is Recombinase | true |
Is Wild Type | false |
molecularNote | By genome editing using CRISPR-Cas9 technology, a cDNA containing loxp-stop-loxp-Dre-polyA (LSL-Dre) cassette was inserted into the translation start codon of the Vim gene. To strengthen the stability of the transcripts, the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was also included following the Dre signal sequence. |