| First Author | Takeshima H | Year | 1995 |
| Journal | EMBO J | Volume | 14 |
| Issue | 13 | Pages | 2999-3006 |
| PubMed ID | 7621815 | Mgi Jnum | J:26886 |
| Mgi Id | MGI:74313 | Doi | 10.1002/j.1460-2075.1995.tb07302.x |
| Citation | Takeshima H, et al. (1995) Ca(2+)-induced Ca2+ release in myocytes from dyspedic mice lacking the type-1 ryanodine receptor. EMBO J 14(13):2999-3006 |
| abstractText | While subtypes 1 and 2 of the ryanodine receptor (RyR) function as intracellular Ca2+ release channels, little is known about the function of the third subtype (RyR-3), first identified in brain. Myocytes from mice homozygous for a targeted mutation in the RyR-1 gene (dyspedic mice) can now be used for a study on the function of RyR-3, which is predominantly expressed in these cells according to our reverse transcription-polymerase chain reaction analysis. We here demonstrate in these myocytes caffeine-, ryanodine- and adenine nucleotide-sensitive Ca(2+)-induced Ca2+ release with approximately 10 times lower sensitivity to Ca2+ than that of RyR-1. Although RyR-3 does not mediate excitation-contraction coupling of the skeletal muscle type, we propose that RyR-3 may induce intracellular Ca2+ release in response to a Ca2+ rise with a high threshold. |
