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Publication : mRNA decapping factor Dcp1a is essential for embryonic growth in mice.

First Author  Ibayashi M Year  2021
Journal  Biochem Biophys Res Commun Volume  555
Pages  128-133 PubMed ID  33813271
Mgi Jnum  J:305571 Mgi Id  MGI:6706030
Doi  10.1016/j.bbrc.2021.03.117 Citation  Ibayashi M, et al. (2021) mRNA decapping factor Dcp1a is essential for embryonic growth in mice. Biochem Biophys Res Commun 555:128-133
abstractText  mRNA decapping is a critical step in posttranscriptional regulation of gene expression in eukaryotes. Although Dcp1a is a well characterized and widely conserved mRNA decapping factor, little is known about its physiological function. To extend our understanding of Dcp1a function in vivo, we employed a transgenic rescue strategy to produce Dcp1a-deficient mice using the CRISPR/Cas9 system. This approach arrowed us to generate heterozygous Dcp1a mice and define the phenotype of Dcp1a-deficient embryos. We found that expression of Dcp1a protein, which is detectable in most mouse tissues, was developmentally regulated through embryonic growth, and that depletion of the Dcp1a gene resulted in embryonic lethality around embryonic day 10.5 (E10.5) concomitant with massive growth retardation and cardiac developmental defects. Moreover, the embryonic lethality was fully rescued by transgenic expression of exogenous human Dcp1a. Together, our results suggest that Dcp1a is required for embryonic growth.
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