| First Author | Tamai Y | Year | 1991 |
| Journal | Gene | Volume | 104 |
| Issue | 2 | Pages | 169-76 |
| PubMed ID | 1717348 | Mgi Jnum | J:11466 |
| Mgi Id | MGI:59899 | Doi | 10.1016/0378-1119(91)90247-9 |
| Citation | Tamai Y, et al. (1991) Sequence of EndoA gene encoding mouse cytokeratin and its methylation state in the CpG-rich region. Gene 104(2):169-76 |
| abstractText | A genomic clone obtained from mouse liver DNA using a mouse cytokeratin EndoA cDNA probe revealed the complete sequence of the EndoA gene. The gene is divided into nine exons and the exon-intron pattern has been conserved compared to that of other type-II cytokeratin-encoding genes. The 5' upstream, 3' downstream and first and third introns contain potential regulatory sequences, including polyoma virus enhancer motifs (PEA1 and PEA3) and AP-1 elements. The 5' regions upstream of the EndoA, EndoB and Ck8 genes contain homologous sequences surrounding the TATA boxes. In addition, a CpG dinucleotide cluster region was located around the first exon. This CpG cluster region was found to be hypomethylated in endodermal PYS-2 cells, retinoic acid-treated F9 cells, and F9 embryonal carcinoma cells, but hypermethylated in BALB/C 3T3 fibroblast cells that do not express EndoA. These findings may provide a clue to understanding the molecular mechanisms of EndoA gene expression. |
