| First Author | Klose RJ | Year | 2007 |
| Journal | Cell | Volume | 128 |
| Issue | 5 | Pages | 889-900 |
| PubMed ID | 17320163 | Mgi Jnum | J:137185 |
| Mgi Id | MGI:3798296 | Doi | 10.1016/j.cell.2007.02.013 |
| Citation | Klose RJ, et al. (2007) The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Cell 128(5):889-900 |
| abstractText | Changes in histone methylation status regulate chromatin structure and DNA-dependent processes such as transcription. Recent studies indicate that, analogous to other histone modifications, histone methylation is reversible. Retinoblastoma binding protein 2 (RBP2), a nuclear protein implicated in the regulation of transcription and differentiation by the retinoblastoma tumor suppressor protein, contains a JmjC domain recently defined as a histone demethylase signature motif. Here we report that RBP2 is a demethylase that specifically catalyzes demethylation on H3K4, whose methylation is normally associated with transcriptionally active genes. RBP2-/- mouse cells displayed enhanced transcription of certain cytokine genes, which, in the case of SDF1, was associated with increased H3K4 trimethylation. Furthermore, RBP2 specifically demethylated H3K4 in biochemical and cell-based assays. These studies provide mechanistic insights into transcriptional regulation by RBP2 and provide the first example of a mammalian enzyme capable of erasing trimethylated H3K4. |
