| First Author | Brown SA | Year | 2005 |
| Journal | Science | Volume | 308 |
| Issue | 5722 | Pages | 693-6 |
| PubMed ID | 15860628 | Mgi Jnum | J:209490 |
| Mgi Id | MGI:5567916 | Doi | 10.1126/science.1107373 |
| Citation | Brown SA, et al. (2005) PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. Science 308(5722):693-6 |
| abstractText | The clock proteins PERIOD1 (PER1) and PERIOD2 (PER2) play essential roles in a negative transcriptional feedback loop that generates circadian rhythms in mammalian cells. We identified two PER1-associated factors, NONO and WDR5, that modulate PER activity. The reduction of NONO expression by RNA interference (RNAi) attenuated circadian rhythms in mammalian cells, and fruit flies carrying a hypomorphic allele were nearly arrhythmic. WDR5, a subunit of histone methyltransferase complexes, augmented PER-mediated transcriptional repression, and its reduction by RNAi diminished circadian histone methylations at the promoter of a clock gene. |
