| Primary Identifier | MGI:5568615 | Allele Type | Targeted |
| Attribute String | Hypomorph | Gene | Gys2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked puromycin resistance cassette was inserted upstream of a modified exon 14 in which a pint mutation results in the amino acid substitution of alanine for arginine at position 582 (R582A). This mutation results in a product that exhibits profoundly impaired activation by glucose-6-phosphate. However, regulation by reversible phosphorylation remains intact. Flp-mediated recombination removed the selection cassette. Western blot analysis confirmed reduced protein expression in the liver. |
