| First Author | Lee SH | Year | 2009 |
| Journal | Biochem Biophys Res Commun | Volume | 381 |
| Issue | 2 | Pages | 214-7 |
| PubMed ID | 19338776 | Mgi Jnum | J:147450 |
| Mgi Id | MGI:3840742 | Doi | 10.1016/j.bbrc.2009.02.026 |
| Citation | Lee SH, et al. (2009) Runx3 inhibits IL-4 production in T cells via physical interaction with NFAT. Biochem Biophys Res Commun 381(2):214-7 |
| abstractText | Interleukin (IL)-4 plays a key role in T helper 2 (Th2) cell differentiation favoring humoral immune response. Regulation of IL-4 gene expression, therefore, is critically important for Th2 dependent responses and Th2 dominant disorders. In T cells, IL-4 gene expression is regulated positively or negatively by a combination of several transcription factors. Recently, enhanced IL-4 production was reported in Runx3 knockout mice; this implies negative regulation of IL-4 by Runx3. Runx proteins are transcription factors that have a Runt domain and have essential functions in development. In this study, the molecular mechanism that downregulates IL-4 expression was investigated. Runx3 inhibited IL-4 production in EL-4 T cells stimulated with PMA/ionomycin. Runx3-mediated IL-4 inhibition was NFAT-dependent, and Runx3 was physically associated with NFAT. Therefore, our results suggest that the interaction between NFAT and Runx3 is a mechanism that causes the negative regulation of IL-4, along with previously reported repression by T-bet. |
