| First Author | Budhram-Mahadeo V | Year | 1996 |
| Journal | J Biol Chem | Volume | 271 |
| Issue | 15 | Pages | 9108-13 |
| PubMed ID | 8621561 | Mgi Jnum | J:235271 |
| Mgi Id | MGI:5795831 | Doi | 10.1074/jbc.271.15.9108 |
| Citation | Budhram-Mahadeo V, et al. (1996) The different activities of the two activation domains of the Brn-3a transcription factor are dependent on the context of the binding site. J Biol Chem 271(15):9108-13 |
| abstractText | The POU (Pit-Oct-Unc) family transcription factor Brn-3a contains two distinct activation domains, one at the N terminus of the molecule and one at the C terminus coincident with the DNA binding domain. These different activation domains have been shown previously to differ in their ability to activate an artificial test promoter containing a Brn-3a binding site and the naturally occurring alpha-internexin gene promoter. Here we identify the target site for Brn-3a in the alpha-internexin gene promoter and show that it can confer responsiveness to Brn-3a on a heterologous promoter. One of the single-stranded DNA sequences derived from either this novel Brn-3a binding site or from the previously characterized site in the test promoter are shown to bind Brn-3a preferentially compared with the complementary single strand or the corresponding double-stranded sequence. The pattern of responsiveness of these two sequences when cloned upstream of the same test promoter and co-transfected with constructs encoding various portions of Brn-3a indicates that the activity of the two Brn-3a activation domains is dependent upon differences in the context of the target sequence in each promoter rather than on differences in the target sequence itself. |
