Primary Identifier | MGI:4949671 | Allele Type | Targeted |
Attribute String | Modified isoform(s) | Gene | Ldb3 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 10. An FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 10. Flp-mediated recombination removed the neo cassette. Cre-mediated recombination removed exon 10, knocking out the short splice variant. The absence of the short form and expression of the long form was confirmed by western blot analysis. |