Primary Identifier | MGI:7516071 | Allele Type | Endonuclease-mediated |
Attribute String | Recombinase, Reporter | Gene | Fcgr1 |
Transmission | Germline | Strain of Origin | C57BL/6 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | The targeting construct inserted IRES, tdTomato, T2A self-cleaving peptide, iCre recombinase, and an FRT site-flanked PGK-NEO-BGHpA cassette downstream of the stop codon of the Fcgr1 gene by CRISPRâCas9-mediated homologous recombination in mouse ES cells. Mice harboring the targeted allele were subsequently crossed with Rosa26FLPe mice to remove the NEO cassette. |