| Primary Identifier | MGI:7448917 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, No functional change | Gene | Braf |
| Transmission | Germline | Strain of Origin | 129/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert a FRT site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a PGK-neo STOP cassette and a second FRT site. A modified exon 15 was engineered into the vector to encode Braf with a valine to glutamate substitution at amino acid 600 (p.V600E) and a silent XbaI restriction site. |
