| First Author | Davy A | Year | 2004 |
| Journal | Genes Dev | Volume | 18 |
| Issue | 5 | Pages | 572-83 |
| PubMed ID | 15037550 | Mgi Jnum | J:89008 |
| Mgi Id | MGI:3037600 | Doi | 10.1101/gad.1171704 |
| Citation | Davy A, et al. (2004) Ephrin-B1 forward and reverse signaling are required during mouse development. Genes Dev 18(5):572-83 |
| abstractText | Eph receptors and ephrin ligands are key players in many developmental processes including embryo patterning, angiogenesis, and axon guidance. Eph/ephrin interactions lead to the generation of a bidirectional signal, in which both the Eph receptors and the ephrins activate downstream signaling cascades simultaneously. To understand the role of ephrin-B1 and the importance of ephrin-B1-induced reverse signaling during embryonic development, we have generated mouse lines carrying mutations in the efnb1 gene. Complete ablation of ephrin-B1 resulted in perinatal lethality associated with a range of phenotypes, including defects in neural crest cell (NCC)-derived tissues, incomplete body wall closure, and abnormal skeletal patterning. Conditional deletion of ephrin-B1 demonstrated that ephrin-B1 acts autonomously in NCCs, and controls their migration. Last, a mutation in the PDZ binding domain indicated that ephrin-B1-induced reverse signaling is required in NCCs. Our results demonstrate that ephrin-B1 acts both as a ligand and as a receptor in a tissue-specific manner during embryogenesis. |
