Primary Identifier | MGI:5661584 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase | Gene | Pip |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | The eGFP-CreERT2 (GCE) fragment with an SV40 poly adenylation site and neomycin resistance cassette were inserted immediately downstream of the translational initiation codon ATG. The knock-in construct removed the coding sequences from exon 1 and placed the GCE gene under the control of the endogenous regulatory sequences. Flp-mediated recombination removed the selection cassette. The EGFP could not be detected by direct fluorescence or anti-GFP antibody staining. |