| First Author | Wu Y | Year | 2013 |
| Journal | FEBS Lett | Volume | 587 |
| Issue | 14 | Pages | 2099-104 |
| PubMed ID | 23707421 | Mgi Jnum | J:198994 |
| Mgi Id | MGI:5500092 | Doi | 10.1016/j.febslet.2013.04.049 |
| Citation | Wu Y, et al. (2013) HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm. FEBS Lett 587(14):2099-104 |
| abstractText | Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, a testis-specific binding protein of HSF2, as a binding partner of BNC1 by using yeast two-hybrid screening. HSF2BP could interact with and inhibit BNC1 transcriptional activity without affecting its expression level. Moreover, coexpression of HSF2BP with BNC1 resulted in a striking redistribution of BNC1 to the cytoplasm. These data suggest that HSF2BP may play a pivotal role in regulating BNC1 transcriptional activity and subcellular localization during spermatogenesis. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: Bnc1physically interacts with HSF2BP by two hybrid (View interaction) Bnc1 and HSF2BPcolocalize by fluorescence microscopy (View interaction) Bnc1physically interacts with HSF2BP by anti tag coimmunoprecipitation (View Interaction: 1, 2). |
