Primary Identifier | MGI:6450020 | Allele Type | Targeted |
Attribute String | Humanized sequence | Gene | Ryr1 |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector is designed to insert an FRT-flanked neomycin resistance gene upstream of exon 34, an amino acid substitution at position 1669 altering serine to cysteine (S1669C; TCG to TGC) and a Leu1716 deletion (CTC) into exon 34. A silent mutation Leu1715 (CTC to CTG) was introduced to create a Bcl1 enzyme site. The neo cassette was deleted by transient transfection of an expression vector of flp recombinase in the ES cells. |