Primary Identifier | MGI:5499291 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Kdm6a |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked STOP cassette with a 3' loxP site was inserted upstream of exon 3. A rox flanked hygromycin resistance (hygro) cassette with a 3' loxP site was inserted downstream of exon 3. Flp- and dre-mediated recombination removed the STOP and hygro cassettes. Cre-mediated recombination removed exon 3. Western blot analysis confirmed the absence of protein expression. |