Primary Identifier | MGI:3799073 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Syt5 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed. |