| First Author | Mohamadzadeh M | Year | 1996 |
| Journal | Eur J Immunol | Volume | 26 |
| Issue | 1 | Pages | 156-60 |
| PubMed ID | 8566059 | Mgi Jnum | J:33089 |
| Mgi Id | MGI:80570 | Doi | 10.1002/eji.1830260124 |
| Citation | Mohamadzadeh M, et al. (1996) Expression of the common cytokine receptor gamma chain by murine dendritic cells including epidermal Langerhans cells. Eur J Immunol 26(1):156-60 |
| abstractText | The common cytokine receptor gamma chain (gamma c) is an indispensable component of interleukin (IL)-2, IL-4, IL-7, IL-9, and IL-15 receptors, and its expression has been detected in several leukocyte populations, including T cells, B cells, monocytes, natural killer cells, and neutrophils. The purpose of this study was to determine whether gamma c receptors are expressed by dendritic cells (DC). Constitutive gamma c mRNA expression was observed by reverse transcription polymerase chain reaction and/or Northern blotting for: (a) Ia+ epidermal Langerhans cells (LC), (b) 4F7+ splenic DC, (c) granulocyte/macrophage colony-stimulated factor-propagated bone marrow-derived DC, and (d) the epidermal-derived DC line, XS52, which retains important functions of epidermal LC. Exposure of XS52 cells to recombinant IL-4 induced a rapid up-regulation of c-myc mRNA expression, and this IL-4-dependent signaling was blocked almost completely by anti-gamma c monoclonal antibody (mAb) TUGm2 in a soluble form. Moreover, c-myc up-regulation was inducible in XS52 cells by the same mAb in an immobilized form. These results imply that molecules recognized by this antibody (i.e. gamma c receptors) are expressed on XS52 cell surfaces. We thus conclude that DC express functional gamma c receptors, which then mediate cytokine-dependent regulation of DC functions. |
