| First Author | Lindsey AE | Year | 1990 |
| Journal | Proc Natl Acad Sci U S A | Volume | 87 |
| Issue | 14 | Pages | 5278-82 |
| PubMed ID | 2371270 | Mgi Jnum | J:331357 |
| Mgi Id | MGI:7387169 | Doi | 10.1073/pnas.87.14.5278 |
| Citation | Lindsey AE, et al. (1990) Functional expression and subcellular localization of an anion exchanger cloned from choroid plexus. Proc Natl Acad Sci U S A 87(14):5278-82 |
| abstractText | We have isolated rat brain cDNA clones encoding AE2, a homologue of the erythrocyte anion exchanger, band 3 (AE1). Immunocytochemistry and in situ hybridization reveal that, in brain, AE2 expression is restricted to the basolateral membrane of the choroid plexus epithelium. Expression of a full-length mouse AE2 cDNA in COS-7 cells resulted in chloride- and bicarbonate-dependent alterations in intracellular pH, demonstrating that AE2 is a Cl/HCO3 exchanger. Cation replacement studies indicate that AE2-mediated exchange is independent of extracellular sodium. COS-7 cells expressing a mutant rat AE2 cDNA clone that lacks the cytoplasmic NH2-terminal 660 amino acids exhibit identical responses to cation and anion substitution. These results indicate that this domain does not play a significant role in either correct insertion of the transporter into the plasma membrane or anion exchange. |
