| First Author | Radomska HS | Year | 1994 |
| Journal | Immunity | Volume | 1 |
| Issue | 8 | Pages | 623-34 |
| PubMed ID | 7600290 | Mgi Jnum | J:28351 |
| Mgi Id | MGI:75969 | Doi | 10.1016/1074-7613(94)90034-5 |
| Citation | Radomska HS, et al. (1994) Constitutively expressed Oct-2 prevents immunoglobulin gene silencing in myeloma x T cell hybrids. Immunity 1(8):623-34 |
| abstractText | Recent experiments involving disruption of the Oct-2 gene have shown that this largely B cell-restricted transcription factor is not required in the early stages of B cell development. However, B cells that lack Oct-2 may be blocked from differentiation past the surface immunoglobulin-positive stage. To identify a possible function for Oct-2 in the late stage immunoglobulin-secreting cell, we have used the method of somatic cell fusion. When the immunoglobulin-producing myeloma MPC11 is fused to a T lymphoma, Oct-2 production ceases, as does the expression of immunoglobulin, J chain, and several other B cell-specific gene products. In the present study, we show that by preventing the loss of Oct-2 in the hybrid cells, we can preserve expression of all other tested B cell-specific genes. These results establish a central role for Oct-2 in maintaining the genetic program of the immunoglobulin-secreting plasmacyte. |
