| First Author | Kim EJ | Year | 2020 |
| Journal | Neuron | Volume | 107 |
| Issue | 2 | Pages | 274-282.e6 |
| PubMed ID | 32396852 | Mgi Jnum | J:292687 |
| Mgi Id | MGI:6449274 | Doi | 10.1016/j.neuron.2020.04.018 |
| Citation | Kim EJ, et al. (2020) Extraction of Distinct Neuronal Cell Types from within a Genetically Continuous Population. Neuron 107(2):274-282.e6 |
| abstractText | Single-cell transcriptomics of neocortical neurons have revealed more than 100 clusters corresponding to putative cell types. For inhibitory and subcortical projection neurons (SCPNs), there is a strong concordance between clusters and anatomical descriptions of cell types. In contrast, cortico-cortical projection neurons (CCPNs) separate into surprisingly few transcriptomic clusters, despite their diverse anatomical projection types. We used projection-dependent single-cell transcriptomic analyses and monosynaptic rabies tracing to compare mouse primary visual cortex CCPNs projecting to different higher visual areas. We find that layer 2/3 CCPNs with different anatomical projections differ systematically in their gene expressions, despite forming only a single genetic cluster. Furthermore, these neurons receive feedback selectively from the same areas to which they project. These findings demonstrate that gene-expression analysis in isolation is insufficient to identify neuron types and have important implications for understanding the functional role of cortical feedback circuits. |
