Primary Identifier | MGI:2180101 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Adh5 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing a PGK-neo gene upstream of exon 5 was used to replace exons 1 through 4. Transcripts were undetectable in total RNA extracts from the liver, kidney, and epididymis of homozygous mutant mice by Northern blot analysis. A lack of protein expression was confirmed by Western blot analysis. |