| First Author | Ueda Y | Year | 2008 |
| Journal | FEBS Lett | Volume | 582 |
| Issue | 10 | Pages | 1459-64 |
| PubMed ID | 18381074 | Mgi Jnum | J:134262 |
| Mgi Id | MGI:3785203 | Doi | 10.1016/j.febslet.2008.03.032 |
| Citation | Ueda Y, et al. (2008) Small mitochondrial ARF (smARF) is located in both the nucleus and cytoplasm, induces cell death, and activates p53 in mouse fibroblasts. FEBS Lett 582(10):1459-64 |
| abstractText | The ARF transcript produces two proteins, the full-length ARF, p19(ARF), and a short mitochondrial version, smARF. To explore the functional difference between the two, we generated GFP-fused expression vectors for each protein and introduced them into NIH3T3 murine fibroblasts, which sustains a global deletion in the INK4a locus but contains a functional p53 gene. GFP-p19(ARF) was located within the nucleolus as previously reported, whereas GFP-smARF was detected mainly in the nucleoplasm. GFP-smARF induced cell death although to a slightly lesser extent than p19(ARF). GFP-smARF stabilized p53 thereby inducing expression of the target genes, MDM2 and p21. We suggest that smARF has functions other than mitochondria-mediated autophagy, and induces p53 expression and cell death via a novel mechanism. |
