| Primary Identifier | MGI:2388492 | Allele Type | Targeted |
| Attribute String | Modified regulatory region | Gene | Igh |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The DQ52 element of Igh-D was disrupted by the insertion of a loxP site flanked neomycin resistance gene cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Ightm6Cgn, this allele contains an unmutated Igh-V11 sequence. The Igh intron enhancer (Rr150) was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. |
