| Primary Identifier | MGI:3611843 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gabra3 |
| Transmission | Germline | Strain of Origin | 129X1/SvJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Insertion vector IV-3KO was used to insert a cassette containing an FRT-flanked neo with a 3' loxP site and a copy of exon 4 with a second loxP site downstream. The duplication of exon 4 was predicted to shift the reading frame and prevent formation of the protein. Crossing with mice expressing FLP recombinase removed the neomycin resistance gene. RT-PCR failed to detect transcript containing the wild-type allele or the duplicate exon. Northern blot, Western blot and immunohistochemical analyses confirmed absence of transcript and protein in mutants, respectively. |
