Primary Identifier | MGI:5294806 | Allele Type | Transgenic |
Attribute String | Inducible, Recombinase | Gene | Tg(Bmi1-cre/ERT2)1Fjs |
Strain of Origin | C57BL/6 | Induced With | tamoxifen |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A BAC (RP23-181D14) containing the Bmi1 locus was modified via recombineering methods by insertion of an IRES-cre/ERT2-pA-Frt-neo-Frt cassette at 85 base pairs 3' of the stop codon of Bmi1. The neo cassette was deleted by transforming the modified BAC into arabinose-induced cells expressing Flp. The final vector was injected into C57BL/6 blastocysts to generate germline-transmitting mice. |