Primary Identifier | MGI:7439917 | Allele Type | Targeted |
Attribute String | Recombinase, Reporter | Gene | Cbln4 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | true | Is Wild Type | false |
molecularNote | A cassette encoding IRES-mnCre:GFP (an internal ribosome entry sequence (IRES), a Myc-tag and nuclear localization signals at the N-terminus of Cre recombinase, which is fused to green fluorescent protein followed by a SV40 polyadenylation sequence) was inserted just 3' of the termination codon in the last coding exon of the Cb;m4 gene via homologous recombination. The neomycin cassette was excised through crosses with animals broadly expressing Flp recombinase. |