| Primary Identifier | MGI:6195533 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Irs1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site and an FRT site flanked neomycin resistance gene cassette were inserted upstream of the gene. An F3 site flanked puromycin resistance gene cassette and a second loxP site were inserted into intron 1, resulting in a distance of around 7 kB between the loxP sites. The neo and puro cassettes were removed through subsequent flp-mediated recombination, leaving a conditional-ready allele with exon 1, containing the entire coding region, floxed. |
