First Author | Salkowski CA | Year | 1992 |
Journal | J Immunol | Volume | 148 |
Issue | 9 | Pages | 2770-7 |
PubMed ID | 1315358 | Mgi Jnum | J:787 |
Mgi Id | MGI:49321 | Doi | 10.4049/jimmunol.148.9.2770 |
Citation | Salkowski CA, et al. (1992) IFN-gamma mediates increased glucocorticoid receptor expression in murine macrophages. J Immunol 148(9):2770-7 |
abstractText | Exposure of the murine macrophage cell line, RAW 264.7, to murine rIFN-gamma resulted in a significant increase in the number of glucocorticoid receptors (GcR). A doubling in the number of GcR was observed as early as 24 h after rIFN-gamma treatment, and receptor number was maximal by 36 h after rIFN-gamma treatment and represented approximately a fourfold increase. Scatchard analysis indicated that a twofold increase in GcR affinity was concomitant with the rIFN-gamma-induced increase in GcR number in RAW 264.7 cells. Increased GcR numbers were induced after exposure of RAW 264.7 cells to as little as 0.1 U/ml rIFN-gamma, and optimal expression was observed at 5 U/ml. Treatment of peritoneal exudate macrophages from C3H/OuJ mice and the LPS hyporesponsive mouse strain, C3H/HeJ, with rIFN-gamma induced an approximately twofold increase in the GcR with no concomitant change in receptor affinity. These results suggest that IFN-gamma may be essential not only for macrophage activation, but also for increasing macrophage sensitivity to feedback inhibition by glucocorticoids by increasing the number and/or affinity of available GcR. |