| First Author | Onishi Y | Year | 2008 |
| Journal | Mol Cell Biol | Volume | 28 |
| Issue | 10 | Pages | 3477-88 |
| PubMed ID | 18332112 | Mgi Jnum | J:136001 |
| Mgi Id | MGI:3794926 | Doi | 10.1128/MCB.02227-07 |
| Citation | Onishi Y, et al. (2008) Rhythmic SAF-A binding underlies circadian transcription of the Bmal1 gene. Mol Cell Biol 28(10):3477-88 |
| abstractText | Although Bmal1 is a key component of the mammalian clock system, little is understood about the actual mechanism of circadian Bmal1 gene transcription, particularly at the chromatin level. Here we discovered a unique chromatin structure within the Bmal1 promoter. The RORE region, which is a critical cis element for the circadian regulation of the Bmal1 gene, is comprised of GC-rich open chromatin. The 3'-flanking region of the promoter inhibited rhythmic transcription in the reporter gene assay in vitro even in the presence of RORalpha and REV-ERBalpha. We also found that the nuclear matrix protein SAF-A binds to the 3'-flanking region with circadian timing, which was correlated with Bmal1 expression by footprinting in vivo. These results suggest that the unique chromatin structure containing SAF-A is required for the circadian transcriptional regulation of the Bmal1 gene in cells. |
