| First Author | Dave RK | Year | 2009 |
| Journal | Exp Cell Res | Volume | 315 |
| Issue | 10 | Pages | 1734-44 |
| PubMed ID | 19268662 | Mgi Jnum | J:150385 |
| Mgi Id | MGI:3850609 | Doi | 10.1016/j.yexcr.2009.02.023 |
| Citation | Dave RK, et al. (2009) CD148/DEP-1 association with areas of cytoskeletal organisation in macrophages. Exp Cell Res 315(10):1734-44 |
| abstractText | In macrophages, tyrosine phosphorylation regulates many signalling pathways leading to growth, differentiation, activation, phagocytosis and adhesion. Protein tyrosine phosphatases (PTPs) represent a biochemical counterbalance to the activity of protein tyrosine kinases, thus regulating the dynamic phosphorylation state of a cell. CD148 is a receptor PTP that is highly expressed in macrophages and is further regulated by pro-inflammatory stimuli. CD148 is normally localised to the plasma membrane of macrophages. Following stimulation with CSF-1 or LPS, there was a re-distribution and concentration of CD148 in areas of membrane ruffling. Treatment of macrophages with anti-CD148 monoclonal antibody inhibited CSF-1-induced macrophage spreading, cytoskeletal re-arrangements and chemotaxis, without affecting cell survival. There were no detectable effects on the CSF-1 receptor-akt signalling pathway. These results are consistent with the hypothesis that CD148 is a regulator of macrophage activity. |
