| First Author | Zhang Q | Year | 1997 |
| Journal | Gene | Volume | 202 |
| Issue | 1-2 | Pages | 31-7 |
| PubMed ID | 9427542 | Mgi Jnum | J:44614 |
| Mgi Id | MGI:1100629 | Doi | 10.1016/s0378-1119(97)00446-0 |
| Citation | Zhang Q, et al. (1997) Molecular cloning and characterization of P113, a mouse SNF2/SWI2-related transcription factor. Gene 202(1-2):31-7 |
| abstractText | A full-length cDNA encoding a 113-kDa transcription factor, named P113, was cloned from mouse preadipocyte line 30A5. P113 binds to a 7-bp consensus TNF-response element and a 30-bp fragment from mouse PAI-1 promoter (-88/-59). Sequence analysis indicates that the P113 is highly homologous to HIP116/HLTF (human) and RUSH-1alpha (rabbit). The sequence homology and the fact that P113 contains seven motifs conserved in many DNA-dependent helicases/ATPases indicate that it is a new member of the SNF2/SWI2 protein family. A cysteine-rich motif, called RING finger, was found close to the C-terminus of P113. The expression pattern of P113 mRNA in rat tissues is significantly different from that of HLTF in human tissues. Affinity-purified P113 has an ATPase activity that is activated by DNA in a sequence-specific manner. Using Northern blot analysis and the PAI-1 promoter/luciferase system, we demonstrated that P113 is a transcription factor that activates the transcription of the PAI-1 gene in 30A5 cells. |
