| Primary Identifier | MGI:6460342 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Akt1 |
| Transmission | Chimeric | Strain of Origin | (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = HG3 - (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1 |
| molecularNote | A G to A nucleotide change at position 49 (c.49G>A) was introduced in exon 3 resulting in a glutamate to lysine substitution at amino acid 17 (p.E17K). A loxP-flanked lacZ and neomycin resistance gene fusion cassette containing a transcription termination signal was inserted upstream of exon 3. Cre-mediated recombination in the ES cells removed the lacZ/neomycin cassette resulting in the expression of the mutant protein. |
