| First Author | Sunahara S | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 268 |
| Issue | 2 | Pages | 590-5 |
| PubMed ID | 10679248 | Mgi Jnum | J:60421 |
| Mgi Id | MGI:1353267 | Doi | 10.1006/bbrc.2000.2189 |
| Citation | Sunahara S, et al. (2000) The oocyte-specific methylated region of the U2afbp-rs/U2af1-rs1 gene is dispensable for its imprinted methylation. Biochem Biophys Res Commun 268(2):590-5 |
| abstractText | Imprinted genes harbor discrete regions which are differentially methylated in gametes; usually the final differential methylation patterns in adults are established during embryogenesis through modifications of the initial methylation patterns in gametes. Previous reports have shown that a 200-bp region termed region II within the CpG island of the mouse imprinted U2afbp-rs gene is methylated in oocytes but not in sperm, suggesting that this region is a center for the propagation of methylated CpGs on the maternal allele and is also a candidate for an imprinting control element. To determine whether region II is required for the imprinted methylation of this gene at the endogenous locus, we generated mice carrying a deletion of this region. We herein show that parental methylation differences still exist in the CpG island on the region II-deleted allele. These findings suggest that region II is dispensable for the imprinted methylation of the U2afbp-rs gene. Copyright 2000 Academic Press. |
