First Author | Sakhneny L | Year | 2018 |
Journal | Diabetes | Volume | 67 |
Issue | 3 | Pages | 437-447 |
PubMed ID | 29246974 | Mgi Jnum | J:258314 |
Mgi Id | MGI:6117908 | Doi | 10.2337/db17-0697 |
Citation | Sakhneny L, et al. (2018) Pancreatic Pericytes Support beta-Cell Function in a Tcf7l2-Dependent Manner. Diabetes 67(3):437-447 |
abstractText | Polymorphism in TCF7L2, a component of the canonical Wnt signaling pathway, has a strong association with beta-cell dysfunction and type 2 diabetes through a mechanism that has yet to be defined. beta-Cells rely on cells in their microenvironment, including pericytes, for their proper function. Here, we show that Tcf7l2 activity in pancreatic pericytes is required for beta-cell function. Transgenic mice in which Tcf7l2 was selectively inactivated in their pancreatic pericytes exhibited impaired glucose tolerance due to compromised beta-cell function and glucose-stimulated insulin secretion. Inactivation of pericytic Tcf7l2 was associated with impaired expression of genes required for beta-cell function and maturity in isolated islets. In addition, we identified Tcf7l2-dependent pericytic expression of secreted factors shown to promote beta-cell function, including bone morphogenetic protein 4 (BMP4). Finally, we show that exogenous BMP4 is sufficient to rescue the impaired glucose-stimulated insulin secretion of transgenic mice, pointing to a potential mechanism through which pericytic Tcf7l2 activity affects beta-cells. To conclude, we suggest that pancreatic pericytes produce secreted factors, including BMP4, in a Tcf7l2-dependent manner to support beta-cell function. Our findings thus propose a potential cellular mechanism through which abnormal TCF7L2 activity predisposes individuals to diabetes and implicates abnormalities in the islet microenvironment in this disease. |