| Type | MGI:General | Description | after 90 min of treatment with 10 uM apoptosis inducer ABT-737 (a BH3 mimetic) and staining with Cy5-annexin V, testicular germ cells from 3-week-old mice fail to show induction of phosphatidylserine (PtdSer) exposure, whereas >80% of similarly treated heterozygous germ cells show PtdSer exposure after 60 min when germ cells are purified by density gradient centrifugation, treated with 5 uM ABT-737 and stained with Fluor 488-annexin V and propidium iodide (PI), PtdSer-positive cells are rarely detected on the cell surface, and, when detected, cells are PI-positive, indicating dead cells that have already undergone secondary necrosis |
