| First Author | Chou HS | Year | 1986 |
| Journal | EMBO J | Volume | 5 |
| Issue | 9 | Pages | 2149-55 |
| PubMed ID | 3490968 | Mgi Jnum | J:23982 |
| Mgi Id | MGI:71777 | Doi | 10.1002/j.1460-2075.1986.tb04478.x |
| Citation | Chou HS, et al. (1986) T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments. EMBO J 5(9):2149-55 |
| abstractText | Both cDNA and genomic clones of the T cell receptor (TCR) alpha- and beta-chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non-functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six-member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7-14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general. |
