| First Author | Vosskuhl K | Year | 2010 |
| Journal | J Immunol | Volume | 185 |
| Issue | 1 | Pages | 119-25 |
| PubMed ID | 20511546 | Mgi Jnum | J:161439 |
| Mgi Id | MGI:4459331 | Doi | 10.4049/jimmunol.0902406 |
| Citation | Vosskuhl K, et al. (2010) Lipopolysaccharide-mediated mast cell activation induces IFN-gamma secretion by NK cells. J Immunol 185(1):119-25 |
| abstractText | Mast cells (MCs) that are well known for their important effector function in IgE-associated immune responses play a key role in innate immune defenses. In this study, we investigate the interaction between MCs and NK cells in vitro and in vivo. We show that mouse bone marrow-derived cultured MCs activated with LPS, polyinosinic-polycytidylic acid, or CpG can stimulate NK cells to secrete increasing concentrations of IFN-gamma. MCs induce a 20-fold increase in IFN-gamma release from NK cells after LPS stimulation. This enhancement of IFN-gamma secretion is cell contact dependent and TNF-alpha independent. Furthermore, we show that this interaction is in part mediated by OX40 ligand on MCs. NK cell-mediated cytotoxicity was not affected by the presence of MCs. Intracellular IFN-gamma levels in splenic NK cells are significantly decreased after i.p. injection of LPS in mast cell-deficient (C57BL/6 Kit(wsh/wsh)) mice in comparison with wild-type mice. In conclusion, our data show for the first time a direct mast cell-dependent NK cell activation. This interaction might play an important role in innate immune defense, as it is dependent on the presence of stimulators relevant in innate immune responses. |
