| First Author | Nabeshima Y | Year | 2005 |
| Journal | FEBS Lett | Volume | 579 |
| Issue | 29 | Pages | 6653-8 |
| PubMed ID | 16298365 | Mgi Jnum | J:103772 |
| Mgi Id | MGI:3610706 | Doi | 10.1016/j.febslet.2005.09.104 |
| Citation | Nabeshima Y, et al. (2005) IL-4 modulates the histamine content of mast cells in a mast cell/fibroblast co-culture through a Stat6 signaling pathway in fibroblasts. FEBS Lett 579(29):6653-8 |
| abstractText | IL-4 plays a crucial role in the pathogenesis of allergic diseases, such as the induction of IgE synthesis and the development of mast cells. To further understand the effect of IL-4 on mast cells in skin, we utilized a mast cell/fibroblast co-culture system as an in vitro model of dermal mast cells. IL-4 induced mast cell growth in the culture with fibroblasts. Immunoblot analysis revealed that IL-4 activated Stat6 in both mast cells and fibroblasts. The over-expression of dominant-negative Stat6 in fibroblasts in the presence of IL-4 decreased the histamine content per mast cell, but not the number of mast cells. In contrast, the over-expression of constitutively-active Stat6 in fibroblasts increased the histamine content per mast cell, indicating that the activation of Stat6 in fibroblasts supports the maturation of mast cells co-cultured with fibroblasts. |
